4-Nitrophenyl α-D-galactopyranoside

Research use only, not for human use.

4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for detecting α-galactosidase activity.

4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for detecting α-galactosidase activity. The amount of released pNP is significantly increased when 4-Nitrophenyl α-D-galactopyranoside is used as substrates.(In Vitro):Using 4-nitrophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 whereas no activity was detected at pH 7.0. The digestion assays using pNP glycopyranosides as artificial substrates show that yielding liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most.

Using 4-nitorophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 whereas no activity was detected at pH 7.0. The digestion assays using 4-nitrophenyl (pNP) glycopyranosides as artificial substrates shows that yieldin liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most.

——

PNP-alpha-D-Gal | PNP-α-D-Gal

Others

Other Targets

Alkyl-Chain| Non-cleavable

——

——

7493-95-0

301.25

C12H15NO8

>98% (HPLC)

In Vitro:?H2O : 16.67 mg/mL (55.34 mM)

O[C@@H]1[C@H](O)[C@@H](O)[C@@H](OC2=CC=C([N+]([O-])=O)C=C2)O[C@@H]1CO

——

(-20℃)

With Ice Pack

≥ 2 years